Open AccessImmunohistological localisation of growth factors in stroma and interstitial gland tissue of goat (Capra hircus) ovary
Author(s) -
Suman Saini,
Rayees Ahmad Bhat
Publication year - 2021
Publication title -
bulgarian journal of veterinary medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.211
H-Index - 10
eISSN - 1311-1477
pISSN - 1313-3543
DOI - 10.15547/bjvm.2316
Subject(s) - stromal cell , paracrine signalling , autocrine signalling , platelet derived growth factor receptor , transforming growth factor , growth factor , biology , ovary , platelet derived growth factor , interstitial cell , tgf alpha , immunohistochemistry , microbiology and biotechnology , endocrinology , cell culture , receptor , cancer research , immunology , biochemistry , genetics
The growth factors platelet derived growth factor (PDGF), transforming growth factor alpha (TGF-α) and transforming growth factor beta (TGF-β) have been demonstrated to stimulate the in vitro proliferation of theca and granulosa cells in different animals. The present study was conducted to localise the growth factors PDGF, TGF-α and TGF-β in different types of interstitial cells and stromal cells of normal cycling goat ovaries. Tissue fixed in formalin was processed through a graded series of alcohols and embedded in paraffin wax. The sections were immunohistochemically stained with antibodies against PDGF, TGF-α and TGF-β. The binding affinity of interstitial cells and stromal cells were observed and photographed. The staining pattern of PDGF, TGF-α and TGF-β was mild to strong in stromal cells. The primary and secondary interstitial cells exhibited varied staining patterns for all studied growth factors. These findings in goat suggests that PDGF, TGF-α, TGF-β were potentially an important autocrine regulator of different cell functions and possibly a paracrine regulator of ovarian cell function at various development stages.