Open AccessMolecular typing of cephalosporin resistant serovars of Salmonella enterica from poultry and farm animals
Author(s) -
Abolfazl Rafati Zomorodi,
M Soltani Rad,
Gh. R. Hashemitabar,
Himen Salimizand
Publication year - 2020
Publication title -
bulgarian journal of veterinary medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.211
H-Index - 10
eISSN - 1311-1477
pISSN - 1313-3543
DOI - 10.15547/bjvm.2196
Subject(s) - cephalosporin , biology , serotype , salmonella enterica , veterinary medicine , cefotaxime , salmonella , cefixime , microbiology and biotechnology , medicine , genetics , antibiotics , bacteria
In this study, the susceptibility to cephalosporins of 74 Salmonella isolates, including 16 isolates from farm animals and 58 isolates from poultry was assessed by the disc diffusion method. ESBL production was evaluated by combined disc diffusion method (CDDM) and double disc synergy test (DDST). The genetic relatedness of isolates was investigated by the Rep-PCR method. The highest prevalence of resistance was observed against cefotaxime (27%) and the least – against cefixime (4%). None of the isolates was ESBL positive. The Rep-PCR generated 54 reproducible fingerprint patterns for all isolates and grouped them in four clusters and six singletons. Due to public health risk of cross contamination, it is important to have sufficient information on the occurrence of these resistant isolates.