Open AccessMolecular detection of Chlamydia psittaci and Chlamydia felis in human keratoconjunctivitis cases
Author(s) -
S. Jazi,
Azam Mokhtari,
Azizollah Ebrahimi Kahrizsangi
Publication year - 2020
Publication title -
bulgarian journal of veterinary medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.211
H-Index - 10
eISSN - 1311-1477
pISSN - 1313-3543
DOI - 10.15547/bjvm.2124
Subject(s) - chlamydia psittaci , keratoconjunctivitis , chlamydia , psittacosis , felis , dna extraction , biology , lymphogranuloma venereum , trachoma , medicine , chlamydia trachomatis , polymerase chain reaction , virology , immunology , pathology , cats , gene , genetics
Given the high incidence of keratoconjunctivitis in Iran (approximately 3.6–53.9%) and low efficiency of clinical diagnostic measures, application of laboratory tests for detection of different keratoconjunctivitis/conjunctivitis causes and determination of their accurate prevalence is essential. In this research, conjunctival samples were collected from 100 patients with keratoconjunctivitis signs referred to an eye hospital of Iran. After DNA extraction, PCR was carried out for detection of Chlamydia psittaci and Chlamydia felis. PCR positive products were further subjected for DNA sequencing. In this study, one sample was Chlamydia psittaci positive and none was positive for Chlamydia felis. There wasn’t a statistically significant relationship between working in the field of veterinary medicine or keeping a pet and Chlamydia psittaci prevalence (P>0.05). This study showed a low rate of chlamydial keratoconjunctivitis and therefore further studies for detection of other causes are necessary.