Open AccessReduction of viral load of avian influenza A virus (H9N2) on SPF eggs and cell line by gamma irradiation
Author(s) -
Shadi Javan,
Farahnaz Motamedi-Sedeh,
Mehrouz Dezfulian
Publication year - 2021
Publication title -
bulgarian journal of veterinary medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.211
H-Index - 10
eISSN - 1311-1477
pISSN - 1313-3543
DOI - 10.15547/bjvm.2019-0094
Subject(s) - embryonated , virus , gamma irradiation , infectious dose , virology , cell culture , avian influenza virus , inoculation , biology , influenza a virus subtype h5n1 , irradiation , microbiology and biotechnology , chemistry , immunology , physics , genetics , nuclear physics
Avian influenza A H9N2 viruses are circulating in domestic poultry worldwide. In this research a low-pathogenic AIV H9N2 was multiplied on MDCK cell line and SPF eggs and irradiated by a Nordian gamma cell instrument. Irradiated and non-irradiated AIV samples were titrated by TCID50 and EID50 methods, respectively. Haemagglutinin antigen was analysed by Haemagglutinin test. Infectivity of irradiated virus samples was determined by cell culture and egg inoculation methods. The virus titration decreased as the dose of gamma radiation increased. AIV proliferation on cell culture can be inactivated by gamma irradiation at a lower dose of gamma-ray (20 kGy) than the virus inactivation on embryonated eggs (30 kGy). The safety test showed complete inactivation of AIV on allantoic fluid with gamma-ray doses: 30 kGy and 20 kGy for virus on MDCK cells after four blind cultures.