Open AccessSimilarities between skin culturable bacterial species of pool frogs (Pelophylax lessonae) and their habitat
Author(s) -
Francesca Paola Nocera,
Anna De Filippis,
Nadia Piscopo,
Lauren A. Esposito,
Luisa De Martino
Publication year - 2021
Publication title -
bulgarian journal of veterinary medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.211
H-Index - 10
eISSN - 1311-1477
pISSN - 1313-3543
DOI - 10.15547/bjvm.2019-0054
Subject(s) - biology , agar , microbiology and biotechnology , aeromonas hydrophila , bacteria , enterococcus , veterinary medicine , agar plate , antibiotic resistance , pseudomonas , zoology , antibiotics , medicine , genetics
The aim of the present study was to investigate the culturable microflora of pool frogs (Pelophylax lessonae) and their belonging aquatic environment. A total of 60 samples (56 frog cutaneous swabs, 4 water samples) were inoculated onto different selective and differential agar plates to isolate Gram-positive and Gram-negative bacteria or yeasts. Microbial investigation of the water hosting frogs was also performed. Isolates were identified by API system and their antibiotic resistance profiles were evaluated by disk diffusion method on Mueller Hinton agar plates. Aeromonas hydrophila and Enterococcus durans were detected in almost all collected samples. Many of the bacterial isolates showed multidrug-resistant profiles. Importantly, this study highlights that skin frog microbiota is correlated to the belonging environment, and, moreover, some isolated bacterial strains resulted to be of interest in animal and public health, since the park was frequented by visitors of all ages.