Open AccessAntagonism of plant growth promoting rhizobacteria against the causal agent of the vascular wilting of tomato
Author(s) -
Narmer Fernando Galeano Vanegas,
Sandra María Marulanda Moreno,
Beatriz Elena Padilla Hurtado,
Javier Guillermo Mantilla Afanador,
Nelson Ceballos-Aguirre,
Gloria María Restrepo Franco
Publication year - 2020
Publication title -
revista colombiana de biotecnologia/revista colombiana de biotecnología
Language(s) - English
Resource type - Journals
eISSN - 0123-3475
pISSN - 1909-8758
DOI - 10.15446/rev.colomb.biote.v22n2.79449
Subject(s) - rhizobacteria , antagonism , biology , fusarium oxysporum , germination , horticulture , microbial inoculant , microbiology and biotechnology , botany , agronomy , bacteria , rhizosphere , inoculation , genetics , receptor , biochemistry
Use of biotechnological potential of native microorganisms as bio-inputs is having a great impact on agricultural systems. Plant Growth Promoting Rhizobacteria (PGPR), in addition to their beneficial effect on plant growth and on the availability of soil elements, also have an antagonistic effect against different pathogens. In this study, growth promotion mechanisms with emphasis on the antagonism of PGPR isolated from sugarcane and tomato crops were evaluated. Antagonism against Fusarium oxysporum f.sp lycopersici (Fol) was determined by dual tests, inhibition of germination and production of chitinases and endoglucanases. 52 isolates were evaluated and according to their results in dual tests 10 were selected for further analysis. Isolate GIBI127 showed the best percentage of Inhibition Germination (IG) of Fol (59.29%). Then, a selection index was calculated using results from gi, dual tests and growth promotion mechanisms to select five best isolates. Finally, these bacteria were evaluated for chitinases and endoglucanases production using Miller´s method. As a result, strain GIBI419 (Burkholderia cepacia) showed a higher production of these enzymes. Selected isolates have antagonistic potential along with plant growth promotion characteristics, which can be used for the development of microbial inoculants which allow the establishment of agricultural systems for tomato cultivation that are sustainable, efficient, and environmentally friendly.