Open AccessThe selective obtaining of amyrins from Amazonian Protium oleoresins
Author(s) -
Rosilene Gomes da Silva Ferreira,
Fernanda Guilhon-Simplicio,
Klenicy Kazumy de Lima Yamaguchi,
Priscila Duarte de Lira,
Tallita Marques Machado,
Márcio Antônio Couto Ferreira,
Valdir Florêncio da Veiga Júnior,
Émerson Silva Lima
Publication year - 2020
Publication title -
revista colombiana de ciencias químico-farmacéuticas/revista colombiana de ciencias quimico-farmaceuticas
Language(s) - English
Resource type - Journals
eISSN - 1909-6356
pISSN - 0034-7418
DOI - 10.15446/rcciquifa.v49n2.89923
Subject(s) - maceration (sewage) , sonication , oleoresin , terpene , chromatography , extraction (chemistry) , chemistry , solvent , hexane , organic chemistry , environmental science , environmental engineering
The oleoresin produced by species of genus Protium sp. is rich in alpha and beta-amyrins, two triterpenes with many pharmacogical activities. Considering the need to make the improved obtainment of these products feasible, this study sought to optimize techniques for the extraction and isolation of amyrins from resin. Two methods of extraction (maceration and sonication) with different solvents were compared to direct isolation from crude resin. The isolation of triterpenes was performed by chromatographic columns and the yields of extracts and fractions were analyzed by analysis of variance. The best extraction solvent for amyrins was hexane for both maceration and sonication methods (38.16±2.06% and 37.67±8.21%, respectively). There was no statistical difference between these methods and the direct method (32.05±2.40%). Additionally, the direct method is cheaper and more environmentally friendly. Thus, this study showed that it is possible to obtain a large quantity of amyrins by means of cheap, fast and ecological methods.