Open AccessTalinum paniculatum leaves with in vitro antimicrobial activity against reference and clinical strains of Staphylococcus aureus interfere in oxacillin action
Author(s) -
Cláudio Daniel Cerdeira,
Jeferson Júnior da Silva,
Manoel Francisco Rodrigues Netto,
Marcelo Fabiano Gomes Boriollo,
Gérsika Bitencourt Santos,
Luciana Maria dos Reis,
Maı́sa Ribeiro Pereira Lima Brigagão
Publication year - 2020
Publication title -
revista colombiana de ciencias químico-farmacéuticas/revista colombiana de ciencias quimico-farmaceuticas
Language(s) - English
Resource type - Journals
eISSN - 1909-6356
pISSN - 0034-7418
DOI - 10.15446/rcciquifa.v49n2.89894
Subject(s) - staphylococcus aureus , minimum inhibitory concentration , broth microdilution , minimum bactericidal concentration , antimicrobial , antibacterial activity , toxicity , microbiology and biotechnology , ethyl acetate , traditional medicine , chemistry , biology , bacteria , chromatography , medicine , organic chemistry , genetics
Propose: We evaluated the antibacterial potential of the crude leaf extract (CLE) and fractions hexane (HX) and ethyl acetate (EtOAc) from Talinum paniculatum alone and in association with oxacillin (OXA) against OXA-resistant Staphylococcus aureus (ORSA, environment isolates) and OXA-sensitive S. aureus (OSSA, ATCC 25923). Furthermore, toxicity tests were performed. Methods: The antibacterial activity was evaluated through checkerboard assay (broth microdilution) to establish the minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC). Toxicity test in mice was assessed. Results: The MIC values for the CLE and its fractions against ORSA and OSSA were in the order of HX (500 μg ml–1) = EtOAc < CLE (4000 μg ml–1). EtOAc and HX presented outstanding antibacterial activities against ORSA, and these fractions were bactericidal toward OSSA. Conversely, the associations between plant product (CLE, EtOAc, or HX) and OXA exhibited no synergistic effects. During these associations, there was an increase in OXA MICs anywhere from 2- to 4092-fold. The CLE presented absence of toxicity at a dose of 5 g kg-1 (in vivo). Conclusion: Although T. paniculatum be a good source of bioactive compounds with antistaphylococcal potential, the researchers should be cautious, since its edible leaf may interfere with OXA therapy (mitigating OXA-induced growth inhibition or killing of S. aureus and enhancing S. aureus resistance).