Association of allele variants of IGF-I, GH and PIT-1 genes with production traits of Birkivska Barvista chicken breed

The article considers the questions about association between allelic variants of insulin-like growth factor I (IGF-I), growth hormone (GH) and pituitary transcription factor-1 (PIT-1) genes with the egg productivity of the Borkovskaya Barvistaya chicken breed. Using classical PCR and PCR-RFLP methods, the PstI-polymorphism in the 5'UTR fragment of the IGF-I gene, HinfI-polymorphism in the promoter fragment of IGF-I gene, AluI-polymorphism in the fourth intron of the GH gene and indel polymorphism in the second intron of the PIT-1 gene were determined. As a result of the studies the significant differences in egg weight values on 30 weeks of life for HinfI-polymorphism in the promoter fragment of the IGF-I gene were revealed. For individuals with the C/C genotype was specific the large values of this index compared to A/A (51,7 ± 0,61 vs. 47.9 ± 0,92 respectively). For PstI-polymorphism in the 5'UTR of the IGF-I gene was shown the prevalence in the number of eggs for 12 weeks of productivity for individuals with the C1C1 genotype compared to C2C2 (65,2 ± 3,77 vs. 60,9 ± 1,33), but in this case the differences are not significantly, what can be caused by a small number of individuals with C1C1 genotype (12). There were no significant differences for other loci.