Видовий склад циркулюючої мікрофлори та її стійкість до антибактеріальних препаратів в умовах ПАФ «Бережниця» Жидачівського району Львівської області

The main issue today is the provision of safe and high-quality livestock products, taking into account all aspects of food production, from farm to table. In order to preserve the young animals, increase the resistance of the immune system of the organism, prevent the influence of infectious pathogens on macroorganism and the environment, studies have been conducted to determine the microflora circulating in the conditions of the economy. In particular, from the point of view of food safety, E. coli is a dangerous factor affecting their quality, causing various diseases in humans and animals. In the body of a healthy bovine animal, Escherichia coli may be present in an admissible concentration of up to 107 CFUs, Escherichia coli haemolitica is normally absent in the animal body. Bacteriological studies included seeding of the test material on the nutrient medium, their identification and the study of antibiotic susceptibility. For the analysis of the sensitivity of microorganisms to medicinal (antibacterial) substances by disco-diffusion method, they used a nutrient medium of AGV, on which the «lawn» was sown analyzing cultures and used discs with antibacterial preparations. According to the results of bacteriological studies of milk samples, it has been determined that 70% of the examined samples of pathogenic microflora have not been isolated. In 30% of the tested samples, the presence of pathogenic microorganisms such as Staphylococcus aureus and Proteus vulgaris in milk samples was established. Bacteriological studies of vaginal exudate taken from cows after the calving have shown that they have Streptococcus epidermidis, Enterobacter cloacae, Escherišhia coli, Proteus vulgaris and fungus of the genus Aspergillus spp. Investigations of the exudate from the nasal passages and the calving of the calves have revealed that Escherichia coli is present in all the samples tested. The results of studies of calf sturgeon revealed the presence of a number of microorganisms: Escherihia coli, Proteus vulgaris, Escherihia coli haemolitica, Staphylococcus haemoliticus, molds of the genus Aspergillus in different percentages. When determining the sensitivity of isolated cultures to antibacterial drugs by the disc diffusion method, it has been established that Staphylococcus and Escherihia coli haemolitica cultures are susceptible to all antibiotics; Proteus vulgaris exhibits resistance to ampicillin, amoxicillin.