Open AccessВизначення чутливості виробничого штаму Bacillus anthracis UA–07 до антибіотиків
Author(s) -
Ірина Олександрівна Рубленко,
Валерій Григорович Скрипник
Publication year - 2017
Publication title -
naukovij vìsnik lʹvìvsʹkogo nacìonalʹnogo unìversitetu veterinarnoï medicini ta bìotehnologìj ìmenì s.z. g̀žicʹkogo
Language(s) - English
Resource type - Journals
eISSN - 2518-1327
pISSN - 2413-5550
DOI - 10.15421/nvlvet7318
Subject(s) - bacillus anthracis , antibiotics , microbiology and biotechnology , antibiotic sensitivity , penicillin , tetracycline , biology , bacteria , genetics
Long-term use of antibiotics leads to antibiotic resistance in pathogens. In addition, there is a possibility of permanent genetic changes in strains due to environmental influences. Therefore there is a need for periodic study vaccine strains for sensitivity to antibiotics in order to study and control of their passport characteristics. The aim of our research was necessary to determine the sensitivity of the strain of Bacillus anthracis UA-07 to antibiotics. The results of studies of sensitivity of strain Bacillus anthracis UA–07 to 50 antibiotics were determined by the size of the diameter of the zones of growth retardation. Zone growth delay of 15 mm was considered a sign of a weak antibiotic sensitivity, zone 15–24 mm – a sign of sensitivity and area of more than 24 mm - a sign of the high sensitivity of the strain to this antibiotic. Lack of stunted growth areas points to the insensitivity of the strain to this antibiotic. The research results show that the strain of Bacillus anthracis UA–07 is highly sensitive to ofloxacin (Of5), ampicillin / sulbactam (A/S10/10), linezolid (LZ30), amoxicillin (AMX30), norfloxacin (NX10), cefalotin (CEP30), tetracycline (TE30). Not affect the development of the studied microorganism antibiotics, cefepime (SPM30), nystatin (NS100U) tsefuroksyn (CXM30) tsefeksym (CFM5), sulfadiazine (SZ300), metronidazole (MT4), cefazolin (Cz30).