ВМІСТ ЖИРНИХ КИСЛОТ В ЛІПІДАХ ФЕТАЛЬНИХ СТОВБУРОВИХ КЛІТИН КОТА

Defined content of fatty acids in lipids cat stem cells derived from fetal primary material. Fetal stem cells (FSCs) cat treated by culturing primary material in the CO2 incubator containing 5% CO2, at a temperature 37°С in DMEM medium with the addition of 15 – 20% fetal bulls serum and 1% antibiotic–antimycotic. When confluent monolayer reached 70 – 80%, the cells are removed from the culture dishes and held subcultivation to reduce the heterogeneity of culture. The resulting stem cells are tested for fatty acid content by gas–liquid chromatography.Determination of fatty acids in lipids fetal stem cells conducted under SOST ISO 5508–2001. Sample preparation was performed according to ISO 150 5509–2002 in our modification. A mixture of methyl esters of fatty acids were analyzed on the gas chromatograph Trace GC Ultra with flame ionization detector for capillary column SPTM –2560, 100 m x 0.25 mm ID, 0.20 μm film (Supelco). Identification of fatty acids was performed using a standard sample Supelco 37 Сomponent FAME Mix. Performed quantitative assessment by spectrum of crystal planes valuation peaks methylated derivatives LCD and determine their content as a percentage of the total content of all the LCD.Investigated that the lipids contained cat FSCs short–, medium– and long–chain fatty acids. In the lipid fetal stem cells found cat 18 Number fatty acids from saturated – most of palmitatе (34.53 ± 0.58%), with monounsaturated – оleate (20.20 ± 0.93%), with polyunsaturated – linoleic acid (6.27 ± 0.01%). Least composed of lipids of cells found cis–8.11.14–eykozatriyenovoyi acid (0.03 ± 0.01%).The total content of saturated fatty acids in the lipid cat FSCs is 67.75, unsaturated fatty acids – 32.25%. Saturation ratio is 2.10. Monoyenic fatty acids identified in the number of 23.19%, and polyenic – 9,06%. The index value n3 fatty acids to n6 in lipids cat FSCs is 0.35.