Open AccessЯКІСТЬ СПЕРМІЇВ ЗА РОЗРІДЖЕННЯ ЕЯКУЛЯТІВ БУГАЇВ ЛАКТОЗО–ЖОВТКОВО–ГЛІЦЕРИНОВИМ СЕРЕДОВИЩЕМ
Author(s) -
S.Y. Kava,
Д. Д. Остапив,
I. Yaremchuk,
Yu. V. Bodnar,
N. V. Kuz’mina
Publication year - 2016
Publication title -
naukovij vìsnik lʹvìvsʹkogo nacìonalʹnogo unìversitetu veterinarnoï medicini ta bìotehnologìj ìmenì s.z. g̀žicʹkogo
Language(s) - English
Resource type - Journals
eISSN - 2518-1327
pISSN - 2413-5550
DOI - 10.15421/nvlvet7030
Subject(s) - semen , sperm , yolk , andrology , lactose , biology , chemistry , biochemistry , food science , medicine
Quality and fertilizing ability of spermatozoa in cryopreserved sperm depends on numerous factors caused by both physiological characteristics and environments dilution of bull ejaculates. So studying physiological characteristics of ejaculates, respiratory activity and redox abillity of sperm, content of lipoproteins, succinate dehydrogenase activity and survival of spermatozoa in sperm diluted by lactose–yolk–glycerine environment.It was found that freshly obtained bull semen can be characterized by: volume – 2.0 – 8.0 ml sperm concentration – 0.28–1.56 × 109 cells / ml. Thus, the respiratory activity of sperm ranges from 0.70–2.02 ng–atom O / min × 0,1 ml of semen and redox ability 0,02–0,14 mV/min × 0.1 ml of semen. It was found that typical freshly obtained bull ejaculates characterize by such content of lipoprotein fractions: chylomicrons (hm) – 26,9 ± 1,93%, lipoproteins with very low density – 10,4 ± 0,44%, low (β–LP) – 18,3 ± 1.84%, high (α–LA) – 17,1 ± 1,09% and very high density – 26,8 ± 1,94%. Dilution of semen by lactose–yolk–glycerin environment leads to a likely (p <0,001) increase in the content of lipoproteins with very low density and reduction in high density lipoprotein fraction. The survival of spermatozoa in sperm diluted by lactose–yolk–glycerin medium varies between 77.1–162.0 hours, and the activity of the enzyme–marker of fertilizing ability succinate dehydrogenase – 5.0–71.7 UI / h × 0,1 ml of semen.