Open AccessПАТОМОРФОЛОГІЧНІ ЗМІНИ В ПЕЧІНЦІ СОБАК ЗА ЕКСПЕРИМЕНТАЛЬНОГО САРКОЦИСТОЗУ
Author(s) -
V. E. Zvorygina,
M. P. Prus,
B.V. Borysevich
Publication year - 2016
Publication title -
naukovij vìsnik lʹvìvsʹkogo nacìonalʹnogo unìversitetu veterinarnoï medicini ta bìotehnologìj ìmenì s.z. g̀žicʹkogo
Language(s) - English
Resource type - Journals
eISSN - 2518-1327
pISSN - 2413-5550
DOI - 10.15421/nvlvet7026
Subject(s) - microtome , h&e stain , sarcocystosis , pathology , histology , evisceration (ophthalmology) , puppy , biology , histopathology , lipofuscin , anatomy , staining , medicine , parasite hosting , sarcocystis , ecology , alternative medicine , world wide web , computer science
Вasic macro– and microscopic changes in the liver of dogs in case of experimental sarcocystosis were determined as a result of our studies. Three clinically healthy puppy aged 2 – 4 months who were subjected to infection by feeding within three days of minced beef hearts, affected by sarcocysts, were selected for the experiment. The animals were subjected to euthanasia at the 7th, 14th and 21st day after infection. Autopsy was performed by partial evisceration in the conventional sequence. To detect microscopic structure of the liver, slices of 7– 10 µm thickness, obtained by the sliding microtome, were stained by hematoxylin and eosin Karazi. For detection of the lipids, frozen sections of thickness 15 – 20 µm, stained by Sudan III, were produced by using freezing microtome. Morphometry was performed due to G. Avtandylov. As a result of the research it was established that liver of all dogs macroscopically had not changed in size, its edges were sometimes sharpened, areas of different sizes and shapes of bluish and gray colors were seen. Macroscopic changes in the liver of dogs in case of experimental sarcocystosis were uncharacteristic. Conducting histology research, venous stasis and total hard granular dystrophy of hepatocytes in liver were determined. In case of experimental sarcocystosis of dogs the possibility of gametogony and sporogony of the causative agent in the liver was established.