Open AccessПОРІВНЯЛЬНА ОЦІНКА КОПРОСКОПІЧНИХ МЕТОДІВ ДІАГНОСТИКИ КРИПТОСПОРИДІОЗУ ВЕЛИКОЇ РОГАТОЇ ХУДОБИ
Author(s) -
Martin Danko,
O. L. Tishyn,
R.V. Khomyak
Publication year - 2016
Publication title -
naukovij vìsnik lʹvìvsʹkogo nacìonalʹnogo unìversitetu veterinarnoï medicini ta bìotehnologìj ìmenì s.z. g̀žicʹkogo
Language(s) - English
Resource type - Journals
eISSN - 2518-1327
pISSN - 2413-5550
DOI - 10.15421/nvlvet7016
Subject(s) - cryptosporidium , giemsa stain , feces , staining , cryptosporidium parvum , veterinary medicine , biology , diarrhea , medicine , microbiology and biotechnology , pathology
Cryptosporidiosis in cattle is a significant problem in most countries with intensive livestock, causing significant economic losses. The disease manifests appetite loss, depression, diarrhea, decreased growth, weight loss, retarded growth, and in some cases – death of sick animals. Equally important in the complex treatment and prevention of bovine cryptosporidiosis owned laboratory diagnostics, which allows early disease promptly detect and identify the pathogen. The article presents data on comparative assessment of three coprological methods of laboratory diagnosis of cryptosporidiosis in cattle. For this purpose, selected 10 samples of feces from calves infected with Cryptosporidium parvum, which added saline and were centrifuged. From the resulting suspension made smears and painted over by azure–eosin (by Giemsa) karbol–fuchsin (by Ziehl–Neelsen) safranine (by Kester). The results of the research identifying the lowest Cryptosporidium oocysts obtained by staining smears azure–eosin. Most effective method of detecting Cryptosporidium oocysts in cattle was staining method strokes by Kester. Average Cryptosporidium oocysts detected by this method was 1.9 times higher than Giemsa method and by 1.4 times – than Ziehl–Neelsen method.