Open AccessІМУНОГІСТОХІМІЧНА ХАРАКТЕРИСТИКА СУБПОПУЛЯЦІЙ ЛІМФОЦИТІВ У СЕЛЕЗІНЦІ КУРЕЙ ПРИ ВАКЦИНАЦІЇ ЇХ ПРОТИ ІНФЕКЦІЙНОГО БРОНХІТУ
Author(s) -
С. В. Гуральська
Publication year - 2016
Publication title -
naukovij vìsnik lʹvìvsʹkogo nacìonalʹnogo unìversitetu veterinarnoï medicini ta bìotehnologìj ìmenì s.z. g̀žicʹkogo
Language(s) - English
Resource type - Journals
eISSN - 2518-1327
pISSN - 2413-5550
DOI - 10.15421/nvlvet7014
Subject(s) - spleen , vaccination , immune system , immunology , immunization , immunohistochemistry , lymphocyte subsets , bronchitis , medicine , biology , andrology , physiology , t cell
In the work the immunohistochemical characterization of subpopulations of СD4+, СD8+, CD45RA+ lymphocytes in the spleen of chickens vaccinated against infectious bronchitis clarified. Immunohistochemical studies have shown that changes in the clusters of immune cells after immunization was certain features in the spleen. Our studies showed that vaccination against infectious bronchitis of chickens in the first, 13 and 33, 83 and 103 day influenced on the change in the percentage of cells in the cluster of СD4+ (helper cells). So, after the introduction of the vaccine on the 8 th day, there was a tendency to increase to 9.23 ± 0,39% against 6,91 ± 0.26% in the control group. But at 20 days there was a significant increase in the number of helpers (of 10.07 ± 0,44% (p < 0.01) against 8.51 ± 0.31% in the control. Early supresion manifestations were recorded on 8th day. There has been a dramatic increase in the number of СD8+ in the experimental group, where the number of cells with the marker were higher than in control more than in two times. So, when studying subpopulations of lymphocyte marker СD8+ installed content control for 9.88 ± 0.38% in Chicks which received the vaccine in this period, the number of СD8+ amounted to 24.99 ± 0,46% (p < 0.001). On the 8th day there was a significant increase in the number of CD45RA+ to 14.22 ± 0.18% (p < 0.001), in control group this indicator amounted to 9.95 ± 0.38% Maximum increase observed on the 40th day, when the CD45RA+ in the group immunized chickens amounted to 14.74 ± 0.29% (p < 0.05).