Open AccessGC-MS analysis of cuticular waxes and evaluation of antioxidant and antimicrobial activity of Chaenomeles cathayensis and Ch. × californica fruits
Author(s) -
Y. V. Lykholat,
Н. О. Хромых,
О. О. Дідур,
Т. V. Sklyar,
Т. А. Holubieva,
T. Y. Lykholat,
K. V. Lavrentievа,
О. В. Ляшенко
Publication year - 2021
Publication title -
regulatory mechanisms in biosystems
Language(s) - English
Resource type - Journals
eISSN - 2520-2588
pISSN - 2519-8521
DOI - 10.15421/022199
Subject(s) - chemistry , antimicrobial , wax , food science , botany , biology , biochemistry , organic chemistry
Fruit extracts of the Chaenomeles species are a rich source of compounds having health-promoting properties, while their distribution between the species and cultivars varies significantly depending on both genotype and environmental threats. This study aimed at discovering antioxidant and antimicrobial potential of the secondary metabolites of fruit and waxes of fruit cuticular of introduced Ch. cathayensis and Ch. × californica plants. The sum of detected polyphenols in the isopropanolic fruit extracts varied slightly between the species, while significant excesses in indices were seen for both species peel extracts as compared to pulp extracts. Antimicrobial assays carried out by disc diffusion method showed notable activity of the fruit peel and pulp extracts of both species against all tested Gram-negative and Gram-positive bacterial strains, and two Candida strains as well. Pseudomonas aeruginosa strain was the most resistant to the action of both fruit extracts, especially peel extracts of Ch. cathayensis fruits. As identified by gas chromatography-mass spectrometry (GC-MS) assays, chloroformic extracts from the fruits of cuticular waxes of Ch. cathayensis and Ch. × californica contained six prevailing fractions: aldehydes, alkanes, alcohols, esters, fatty acids and various terpenoids. The predominant compounds were tetrapentacontane (21.8% of total amount) and heptacosanal (23.1% of total), respectively in the cuticular waxes of Ch. cathayensis and Ch. × californica. Cinnamaldehyde, cis-9-hexadecenal, hexadecanoic acid, oleic acid, olean-12-ene-3,28-diol (3. beta), lupeol, diisooctyl phthalate, 9-octadecenoic acid, 1,2,3-propanetriyl ester, 1,3,12-nonadecatriene-5,14-diol and some other identified compounds are well-known for their bioactivity, indicating the feasibility of studying the antimicrobial potential of plant fruits.