Open AccessExpression of CD34 marker in the adipose tissue derived stem cells
Author(s) -
Phuc Van Pham,
Ngọc Bich Vu,
Van Tran
Publication year - 2018
Publication title -
progress in stem cell
Language(s) - English
Resource type - Journals
ISSN - 2199-4633
DOI - 10.15419/psc.v5i1.399
Subject(s) - cd34 , mesenchymal stem cell , stem cell , adipose tissue , haematopoiesis , flow cytometry , stem cell marker , bone marrow , biology , pathology , microbiology and biotechnology , immunology , medicine , endocrinology
Adipose-derived stem cells (ADSCs) are considered as mesenchymal stem cells (MSCs). Indeed, they display all characteristics of MSCs that compliant with the minimal criteria of MSCs suggested by Domonici et al. (2006). However, some recent studies showed that ADSCs contain the subpopulation that was positive with CD34 marker – a marker of hematopoietic stem cells. This study aimed to analyze and determine the expression of CD34 marker in ten samples of ADSCs obtained from 10 donors.
Methods: All ADSC samples were isolated and expanded according to the published previous protocols. They were confirmed as the MSCs with some markers and differentiation potential, excepting the CD34 expression. Then they were cultured and analyzed the expression of CD34 by flow cytometry at passage 3, 5, 7 and 9.
Results: The results showed that expression of CD34 in ADSCs was different between donors and their passages that accounted from 1.21% to 23.38%.
Conclusion: These results suggested that ADSCs are not ‘truly” MSCs like MSCs from bone marrow.