Open AccessPost-Cryo Survival of Rat Testicular Interstitial Cells in Hydroxyethyl Starch-Based Media
Author(s) -
Oleksandr Pakhomov
Publication year - 2021
Publication title -
problemy kriobiologii i kriomediciny
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.189
H-Index - 4
eISSN - 2518-7376
pISSN - 2307-6143
DOI - 10.15407/cryo31.01.070
Subject(s) - dimethyl sulfoxide , cryopreservation , hydroxyethyl starch , bovine serum albumin , fetal bovine serum , andrology , transplantation , chemistry , biochemistry , cell , biology , microbiology and biotechnology , embryo , surgery , medicine , organic chemistry
The development of cryoprotective serum-free or xeno-free media is required for safe use of cryopreserved testicular material for transplantation. In this study, the solutions containing 10% fetal bovine serum (FBS) or 5 mg/ml bovine serum albumin (BSA) did not significantly enhance the general survival of interstitial cells (ICs) after cryopreservation but increase their metabolic activity and steroid producing cell (HSD+-cells) survival. The use of 50 and 100 mg/ml hydroxyethyl starch (HES) in DMSO-based cryoprotective solutions instead of BSA or FBS enabled the improvement of the IC general survival and the survival of HSD+-cells. The use of HES supplemented media allowed to decrease the dimethyl sulfoxide (DMSO) concentration from 1.4 to 0.7 M and to preserve the amount and metabolic activity of ICs. Thus, designing cryoprotective media containing DMSO and HES can facilitate the formulation of serum-free solutions for cryopreservation that in turn paves a way for implementation of the use of cryopreserved material for practical medicine.