Open AccessPolymorphism of TLR1, TLR4, and SLC11A1 genes in populations of different cattle breeds of Ukrainian selection
Author(s) -
Roman Kulibaba,
Yu. V. Liashenko,
OKSANA IVASHCHENKO
Publication year - 2021
Publication title -
agricultural science and practice
Language(s) - English
Resource type - Journals
eISSN - 2312-3389
pISSN - 2312-3370
DOI - 10.15407/agrisp8.03.025
Subject(s) - breed , genetics , genotype , biology , locus (genetics) , allele , population , polymorphism (computer science) , genotype frequency , restriction fragment length polymorphism , exon , gene polymorphism , allele frequency , gene , demography , sociology
Aim. To study the specificities of population genetic structure of Ukrainian Black-and-White dairy breed, UkrainianRed-and-White dairy breed, and Ukrainian Grey cattle breed by polymorphism of TLR1, TLR4, and SLC11A1 genes.Methods. The study was conducted using the method of polymerase chain reaction and restriction fragment lengthpolymorphism (PCR-RFLP). Results. The results of the study demonstrated that in all the experimental populations,the locus TLR4 by mutations 8732G>A, 8834G>C, and 2021C>T was monomorphic, while loci TLR1 (1596G>A)and SLC11A1 (7400C>G and 7808A>T) were polymorphic. For the TLR4 gene by 8732G>A mutation, only individu-als with genotype BB were found; by 8834G>C – with genotype GG; by 2021C>T – with genotype CC. By BclI-polymorphism in the first exon of TLR1 (1596G>A) the A and G allelic variants were found in each experimentalpopulation. The deviation from the Hardy-Weinberg equilibrium state was revealed for the populations of UkrainianBlack-and-White and Ukrainian Grey cattle breeds. A considerable excess of heterozygous individuals was fixed inboth cattle populations (31 and 39 % respectively). Considering PstI-polymorphism in exon 11 of SLC11A1 gene,allelic variants C and G were found for SNP5 (7400C>G), as well as A and T for SNP6 (7808A>T) in all experimen-tal populations. As for SNP5 (7400C>G), a considerable excess (from 15 to 30 %) of heterozygous individuals wasdetermined for all experimental groups. Unlike other breeds, there was no excess (Fis = 0,04) of heterozygotes forSNP6 (7808A>T) in the population of Ukrainian Grey cattle. Conclusions. The parameters of genetic variability inthe different cattle populations of Ukrainian selection by TLR1, TLR4, and SLC11A1 loci were studied. The resultsof the study showed the impossibility of using TLR4 locus by 8732G>A, 8834G>C and 2021C>T mutations in theprograms of marker-associated selection of the investigated cattle breeds due to its monomorphic nature. The analysisof the allele and genotype distribution by TLR1 and SLC11A1 loci (presence of individuals with different genotypes inall experimental cattle breeds) indicates the possibility of using different allelic variants of these genes in the breedingprograms for the studied cattle populations.