Open Access
Formation of Nonspecifi c Resistance in Winter Wheat Plants by the Hydrogen Peroxide Treatment
Author(s) -
L. Batsmanova,
М. М. Musienko
Publication year - 2014
Publication title -
agricultural science and practice
Language(s) - English
Resource type - Journals
eISSN - 2312-3389
pISSN - 2312-3370
DOI - 10.15407/agrisp1.02.033
Subject(s) - lipid peroxidation , catalase , hydrogen peroxide , chemistry , superoxide dismutase , antioxidant , malondialdehyde , thiobarbituric acid , biochemistry , horticulture , food science , botany , biology
Aim. The aim of the investigation was to study the effect of hydrogen peroxide on the activity of antioxidant enzymes, pigments’ content and productivity of winter wheat in order to determine the biochemical mechanisms of induced winter wheat plant resistance during ontogenesis in the fi eld. Methods. The study of physiological and biochemical parameters were carried out on winter wheat varieties of the wooded steppe (Poliska 90) and steppe (Skala) ecotypes at the tillering and fl owering phases. The experimental plants were foliar treated with hydrogen peroxide (Н 2 О 2 ) in concentration of 1∙10 –1 M twice, with a 3-day-interval, while the control ones – with distilled water. Spraying of plants was performed at spring (tillering phase). Lipid peroxidation (LPO) was determined as the formation of a peroxidation end product — malondialdehyde, which content was determined as the extinction of its condensation product with thiobarbituric acid. The activity of superoxide dismutase (SOD) was determined by amethod based on the ability of SOD to inhibit nitroblue tetrazolium recovery by superoxide radicals in the light in the presence of ribofl avin and methionine. The activity of catalase was calculated using the extinction coeffi cient of − 39.4 mM –1 cm –1 . Results. It was established that after 24 h of hydrogen peroxide treatment the processes of lipid peroxidation were intensifi ed and antioxidant enzymes’ activity was decreased in two varieties. In the next phase of ontogenesis (fl owering) antioxidant enzymes’ activity increased in both varieties: Poliska 90 and Skala, which suppressed the LPO growth. Conclusion. Treatment of plants with hydrogen peroxide promoted the formation of non-specifi c plant resistance and increased grain productivity of winter wheat varieties investigated.