Open AccessComparative Study of Highly Pathogenic Avian Infl uenza Strains Isolated in Ukraine in 2005 and 2008
Author(s) -
Anton Gerilovych,
B. Т. Stegniy,
Anton Stegniy,
M. Yu. Stegniy,
Krzysztof Śmietanka,
Ze Minta
Publication year - 2014
Publication title -
agricultural science and practice
Language(s) - English
Resource type - Journals
eISSN - 2312-3389
pISSN - 2312-3370
DOI - 10.15407/agrisp1.01.068
Subject(s) - influenza a virus subtype h5n1 , highly pathogenic , biology , phylogenetic tree , virology , polymerase chain reaction , clade , microbiology and biotechnology , genetics , gene , virus
Objective. To research the molecular characteristics of two HPAI strains – A/Ch/Syvash/02/05/H5N1 and A/Ch/Krasnogvardeysk/58/08/H5N1, which were identifi ed as representatives of the highly pathogenic H5N1 viruses. Methods. RNA extraction, real-time polymerase chain reaction (PCR). Results. The phylogenetic studies revealed that the above mentioned strains belong to two various genetic lineages originated from the Eastern European strains isolated in 2005, but differ from the viruses introduced to the Central and Western Europe in 2005/2006, and also the lineages consisting of H5N1 viruses isolated in the Europe and Middle East in late 2007. Conclusions. Relying on experimental studies, it can be concluded that the strains of A/Ch/Syvash/02/05/H5N1 and A/Ch/Krasnogvardeysk/58/08/H5N1 are highly pathogenic.