Open AccessGenotoxicity and Cytotoxicity of Multi‐wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers
Author(s) -
Asakura Masumi,
Sasaki Toshiaki,
Sugiyama Toshie,
Takaya Mitsutoshi,
Koda Shigeki,
Nagano Kasuke,
Arito Heihachiro,
Fukushima Shoji
Publication year - 2010
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.l9150
Subject(s) - genotoxicity , cytotoxicity , chrysotile , micronucleus test , micronucleus , chinese hamster , chromosome aberration , hamster , chemistry , sonication , neutral red , microbiology and biotechnology , biophysics , biochemistry , materials science , biology , in vitro , toxicity , chromatography , organic chemistry , chromosome , composite material , asbestos , gene
Genotoxicity and Cytotoxicity of Multi‐wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers: Masumi A sakura , et al . Japan Bioassay Research Center, Japan Industrial Safety and Health AssociationObjectives The potential applications and industrial production of multi‐wall carbon nanotubes (MWCNT) have raised serious concerns about their safety for human health and the environment. The present study was designed to examine the in vitro cytotoxicity and genotoxicity of MWCNT and UICC chrysotile A (chrysotile). Methods Cytotoxicity using both colony formation and lactate dehydrogenase (LDH) assays and genotoxicity including chromosome aberration, micronucleus induction and hgprt mutagenicity were examined by exposing cultured Chinese hamster lung (CHL/IU) cells to MWCNT or chrysotile at different concentrations. Results The in vitro cytotoxicity of MWCNT depended on the solvent used for suspension of MWCNT and ultrasonication duration of the MWCNT suspension. A combination of DMSO/culture medium and 3‐minute ultrasonication resulted in a well‐dispersed medium with dispersion and isolation of agglomerated MWCNT by ultrasonication which manifested the highest cytotoxicity. The cytotoxicity was more potent for chrysotile than MWCNT. The genotoxicity of MWCNT was characterized by the formation of polyploidy without structural chromosome aberration, and an increased number of bi‐ and multi‐nucleated cells without micronucleus induction, as well as negative hgprt mutagenicity. Chrysotile exhibited essentially the same genotoxicity as MWCNT, except for marginal but significant induction of micronuclei. MWCNT and chrysotile were incompletely internalized in the cells and localized in the cytoplasm. Conclusions MWCNT and chrysotile were cytotoxic and genotoxic in Chinese hamster lung cells, but might interact indirectly with DNA. The results suggest that both test substances interfere physically with biological processes during cytokinesis.