Open AccessThe Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature
Author(s) -
Matsumoto Yuki,
Ogawa Yasutaka,
Yoshida Rie,
Shimamori Ayako,
Kasai Hiroshi,
Ohta Hisayoshi
Publication year - 2008
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.l7144
Subject(s) - urinary system , 8 hydroxy 2' deoxyguanosine , deoxyguanosine , urine , oxidative stress , chemistry , medicine , urology , endocrinology , dna oxidation , oxidative damage
The Stability of the Oxidative Stress Marker, Urinary 8‐hydroxy‐2'‐ deoxyguanosine (8‐OHdG), when Stored at Room Temperature: Yuki M atsumoto , et al . National Institute of Occupational Safety and Health —We examined the stabilities of urinary 8‐hydroxy‐2'‐deoxyguanosine (8‐OHdG) stored at room temperature (25°C) for 24 h or at −80°C for 800 days. Subjects were 19 males and 17 females aged 23–58 yr for the 24‐h study, and 9 males and 4 females aged 24–54 yr for the 800‐day study. We obtained information on the subjects by questionnaires and interviews. The level of urinary 8‐OHdG was measured by HPLC using two‐step separations. There were no significant changes of amount of urinary 8‐OHdG under either storage conditions. We conclude that urine samples can be stored at 25°C and below for 24 h, when the research purpose includes the determination of urinary 8‐OHdG. Urinary 8‐OHdG was also stable for over two years when stored at −80°C.