Open AccessAcute Arsine Poisoning Confirmed by Speciation Analysis of Arsenic Compounds in the Plasma and Urine by HPLC‐ICP‐MS
Author(s) -
Yoshimura Yukihiro,
Endo Yoko,
Shimoda Yasuyo,
Yamanaka Kenzo,
Endo Ginji
Publication year - 2011
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.l10108
Subject(s) - arsine , arsenite , urine , arsenate , arsenic , chemistry , arsenic poisoning , creatinine , chromatography , environmental chemistry , biochemistry , catalysis , organic chemistry , phosphine
Acute Arsine Poisoning Conirmed by Speciation Analysis of Arsenic Compounds in the Plasma and Urine by HPLC‐ICP‐MS: Yukihiro Yoshimura, et al. Department of Infectious Disease, Yokohama Municipal Citizen's Hospital—Objectives Arsine, a potent hemolytic agent, is widely used in the semiconductor industry. We report a case of arsine poisoning conirmed by arsenic speciation analysis in serum and urine that occurred in a recycling factory. Case A male worker in his twenties noticed hematuria 3 h after inishing work and was admitted into our hospital 34 h later. Speciation analysis of arsenics in serum and urine samples was performed using HPLCICP‐MS. On admission, anemia, hematuria, and renal and liver dysfunction were observed. His clinicalcondition had improved remarkably after 5‐days of transfusion and 4 units of RBC transfusion. The total arsenic content in the serum was 244.8 µg/l at admission and 97.1 µg/l at discharge. In the speciation analysis, four kinds of As compounds derived from arsine metabolism were detected in serum and urine. The concentrations of arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in serum at admission were 45.8, 5.2, 17.9 and 9.3 µg/l, respectively. The concentrations of AsIII, AsV and MMA decreased with biological half‐lives (BHL) of 30.1, 43.0, and 96.3 h, respectively, while that of DMA was increased towards discharge. The urinary AsIII, AsV, MMA, and DMA concentrations at discharge were384.5, 20.8, 547.4 and 1816.3 µg/g creatinine, respectively. These concentrations in urine subsequently decreased and their BHL was 15 days. Conclusion The results of the present study suggest that arsine is quickly metabolized to AsCII and then metabolized via MMA to DMA in humans.