Open AccessExpression of Heme Oxygenase‐1 in the Lungs of Rats Exposed to Potassium Octatitanate Whiskers
Author(s) -
Obata Yasuko,
Morimoto Yasuo,
Hirohashi Masami,
Ogami Akira,
Oyabu Takako,
Myojo Toshihiko,
Kawanami Shoko,
Horie Seichi,
Nagatomo Hiroko,
Murakami Masahiro,
Tanaka Isamu
Publication year - 2011
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.l10056
Subject(s) - heme oxygenase , lung , oxidative stress , andrology , heme , pathogenesis , whiskers , messenger rna , chemistry , immunostaining , gene expression , respiratory system , medicine , endocrinology , biology , gene , immunohistochemistry , biochemistry , enzyme , ecology
Expression of Heme Oxygenase‐1 in the Lungs of Rats Exposed to Potassium Octatitanate Whiskers: Yasuko Obata, et al. Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan—Objectives Oxidative stress is thought to be the pathogenesis of pulmonary ibrosis induced by asbestos, and heme oxygenase‐1 (HO‐1) protects lung tissue against oxidative stress. We hypothesized that HO‐1 is also associated withoxidative lung injury caused by exposure to potassium octatitanate whiskers (PT1), which is one of the asbestos substitutes. Methods Male Wistar rats were administered 1 mg or 2 mg PT1 suspended in saline by a single intratracheal instillation and were sacrificed after recovery for 3 days, 1 wk, 1 mo, 3 mo or 6 mo. Gene expression of HO‐1 protein and mRNA and immunostaining were investigated in rat lungs. Results HO‐1 protein expression was increased from 3 days to1 mo and at 6 mo in the 1 or 2 mg PT1‐exposed groups, and the gene expression of HO‐1 mRNA was also increased at 3 days and from 1 mo to 6 mo. HO‐1positive cells were mainly found in the alveolar macrophages and the bronchial epithelial cells inimmunostaining. Conclusions These indings suggest that HO‐1 is involved in lung damage caused by PT1.