Open AccessOxidative DNA Damage Estimated by Plasma 8‐hydroxydeoxyguanosine (8‐OHdG): Influence of 4, 4'‐methylenebis (2‐chloroaniline) Exposure and Smoking
Author(s) -
Chen HongI,
Liou SaouHsing,
Ho ShuFen,
Wu KuenYuh,
Sun ChienWen,
Chen MingFong,
Cheng LiChuan,
Shih TungSheng,
Loh ChingHui
Publication year - 2007
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.49.389
Subject(s) - urinary system , urine , confounding , carcinogen , medicine , chemistry , dna damage , oxidative phosphorylation , endocrinology , creatinine , oxidative damage , physiology , oxidative stress , dna , biochemistry
Oxidative DNA Damage Estimated by Plasma 8‐hydroxydeoxyguanosine (8‐OHdG): Influence of 4, 4'‐methylenebis (2‐chloroaniline) Exposure and Smoking: Hong‐I C hen , et al . Division of Urology, Department of Surgery, Tri‐Service General Hospital, Taiwan, R.O.C —Oxidative DNA damage may play an important role in the human carcinogenic process. Recently, we reported a case of bladder cancer among 4, 4’‐methylenebis (2‐chloroaniline) (MBOCA)‐exposed workers. By measuring the plasma level of 8‐hydroxydeoxyguanosine (8‐OHdG), we investigated the association between oxidative DNA damage and MBOCA exposure. In addition, we examined the effects of different confounders on the plasma level of 8‐OHdG. We undertook a cross‐sectional survey at four MBOCA‐producing factories in Taiwan (158 subjects). Plasma 8‐OHdG levels and urinary MBOCA concentrations were measured by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Personal characteristics were collected by questionnaire. The workers were classified according to their job titles as exposed (n=57) or unexposed (n=101) groups as well as classified according to urinary MBOCA levels as high urinary MBOCA (>20 µg/g creatinine) (n=45) or low urinary MBOCA (n=108) groups. Neither the MBOCA‐exposed workers nor the high urinary MBOCA workers had a significant increase in the mean plasma 8‐OHdG level, even after adjustment for potential confounders. Age and gender were significantly positively correlated with plasma 8‐OHdG levels. Smokers among high urinary MBOCA workers also had significantly higher 8‐OHdG levels than non‐smokers among high urinary MBOCA workers. Our study provides evidence that smoking rather than MBOCA exposure induces elevation of plasma 8‐OHdG levels among workers exposed to MBOCA, indicating that oxidative DNA damage does not play an important role in the carcinogenic processes of MBOCA.