Open AccessExpression of Clara Cell Secretory Protein in the Lungs of Rats Exposed to Crystalline Silica In Vivo
Author(s) -
Morimoto Yasuo,
Nagatomo Hiroko,
Hirohashi Masami,
Oyabu Takako,
Ogami Akira,
Yamato Hiroshi,
Kuroda Kaori,
Obata Yasuko,
Higashi Toshiaki,
Tanaka Isamu
Publication year - 2005
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.47.504
Subject(s) - in vivo , protein expression , cell , microbiology and biotechnology , chemistry , secretory protein , secretion , biology , biochemistry , gene , genetics
Expression of Clara Cell Secretory Protein in the Lungs of Rats Exposed to Crystalline Silica In Vivo: Yasuo Morimoto, et al. Department of Occupational Pneumology, Institute of Industrial and Ecological Sciences, University of Occupational and Environmental Health —It has been theorized that Clara cell secretion protein (CCSP) plays a critical role in regulating the acute inflammatory response in the lung. We hypothesized that CCSP is also related to lung injury induced by occupational dust. The present study was conducted to investigate the time course of the expression of CCSP in lungs exposed to crystalline silica in vivo. Male Wistar rats were administered 1 mg or 2 mg of silica suspended in saline by a single intratracheal instillation and were sacrificed at 3 d, 1 wk, 1 month, 3 months and 6 months of recovery time. The expression of CCSP was observed by RT‐PCR and western blot analysis. Exposure to 2 mg of silica decreased in levels of CCSP mRNA at 3 d, 1 wk, 1 month and 6 months following intratracheal instillation. The protein level of CCSP in silica‐exposed rats was decreased at 3 d, 7 d and 1 month after a single instillation of 2 mg. The decreases in CCSP at the acute phase in this experiment suggest that CCSP may regulate the acute injury of the lung exposed to silica.