Open AccessOccupational Exposure to Aromatic Hydrocarbons at a Coke Plant: Part I. Identification of Hydrocarbons in Air and their Metabolites in Urine by a Gas Chromatography‐Mass Spectrometry Method
Author(s) -
Bieniek Grażyna,
Kurkiewicz Sławomir,
Wilczok Tadeusz
Publication year - 2004
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.46.175
Subject(s) - silylation , chemistry , chromatography , gas chromatography , trimethylsilyl , gas chromatography–mass spectrometry , carbon disulfide , organic chemistry , extraction (chemistry) , urine , mass spectrometry , aromatic amine , environmental chemistry , catalysis , biochemistry
Occupational Exposure to Aromatic Hydrocarbons at a Coke Plant: Part I. Identification of Hydrocarbons in Air and their Metabolites in Urine by a Gas Chromatography‐Mass Spectrometry Method: Grażyna Bieniek, et al. Department of Molecular Biology, Biochemistry and Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Poland —A method for the qualitative analysis of aromatic hydrocarbons in air and their various urinary metabolites is presented. The air was sampled in charcoal tubes and extracted with carbon disulfide. The hydrocarbons were identified as being aliphatic hydrocarbons (C 9 –C 19 ), aromatic hydrocarbons and heterocyclic compounds. The urinary metabolites after enzymatic hydrolysis were analyzed by solid‐phase extraction with a styrene‐divinylbenzene resin, silylation with N,Obis(trimethylsilyl)acetamide and GC/MS for separation and detection. Satisfactory separation of all compounds investigated was achieved without interference due to matrix peaks. The following compounds were identified in the urine of workers: dimethylphenol isomers, 4‐ethyl‐1,3‐benzenediol, 2ethoxybenzoic acid and methoxyphenols. Trimethylsilyl derivatives of aromatic hydroxyacids and hydroxymethoxyacids were found in the urine of occupationally exposed workers by means of a silylation procedure.