Open AccessDoes the Exposure to 2,5‐Hexanedione Hasten the Onset of Peripheral Neuropathy in Streptozotocin‐Induced Diabetic Rats?
Author(s) -
Goto Toshio,
Kimura Takashi,
Zhao Wenyuan,
Misumi Junichi
Publication year - 1997
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.39.156
Subject(s) - hygiene , public health , medicine , emergency department , family medicine , gerontology , environmental health , nursing , pathology
g (mean ± SD), were randomly allocated to diabetic and normal (non-diabetic) groups (12 in each group). The rats allocated to the diabetic group were injected intraperitoneally with streptozotocin at a dose of 60 mg/kg to induce diabetes. Two weeks after the injection, 11 rats whose nonfasting serum glucose concentrations were higher than 250 mg/dl were assigned to the diabetic group. Five diabetic rats were subcutaneously injected in the back with 2,5-HD (100 mg/ kg/day) dissolved in 0.2 ml of 0.9% NaCl solution, five days a week, for 6 weeks (group DM + HD). The remaining 6 diabetic rats were injected with 0.2 ml of 0.9% NaCI solution alone and allocated as diabetic controls (group DM). The 12 normal non-diabetic rats were also subdivided into two groups, one group of 6 rats were treated with 2,5-HD (100 mg/kg/ day) in the same manner as the DM + HD group rats (group HD), and the remaining 6 rats were injected with 0.2 ml of 0.9% NaCI solution alone and allocated to the control group. The rats were fed CE-2 rat chow (Clea Company Japan) and water ad libitum. Electrophysiological examinations The measurements of motor nerve conduction velocity