Open AccessStandardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications
Author(s) -
Nairy Rajesha K,
Yerol Narayana,
Bhat Nagesh N,
Desai Utkarsha,
Shirsath Kapil,
Yadav Usha,
Chaurasia Rajesh K.,
Sapra BK
Publication year - 2016
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.16-0049-oa
Subject(s) - okadaic acid , biodosimetry , calibration curve , chemistry , yield (engineering) , radiochemistry , irradiation , materials science , chromatography , physics , biochemistry , detection limit , phosphatase , nuclear physics , enzyme , ionizing radiation , metallurgy
Objective In the present study an attempt was made to estimate coefficients of dose response curves for PCC aberrations induced by CalyculinA and Okadaic acid, using 60 Co gamma radiation and 8 MeV pulsed electron beam for biodosimetry application. Materials and Methods The modified method outlined by Puig et al. 2013 was used to conduct Calyculin A and Okadaic acid induced PCC assay in human blood lymphocytes. Chemical treatment was given for the last 1 h of a 48 h culture. The study was carried out in the dose range 2.5 to 20 Gy using 60 Co gamma rays and 8 MeV pulsed electron beam. Results and Conclusions Results show a linear dose dependent increase with a slope of 0.047 ± 0.001 from Calycalin A PCC and 0.048 ± 0.002 form Okadaic acid PCC. The slope of the fragments curve was 0.327 ± 0.006 from Calyculin A and 0.328 ± 0.006 from Okadaic acid PCC. Further, dose calibration studies were carried out for 8 MeV electron using Calyculin A PCC assay and the obtained slope from ring yield was 0.054 ± 0.002 and 0.427 ± 0.009 from fragment yield.