Open AccessEvaluation of DNA damage in lymphocytes of radiology personnel by comet assay
Author(s) -
Khisroon Muhammad,
Khan Ajmal,
Naseem Maryam,
Ali Naheed,
Khan Sardar,
Rasheed Syed Basit
Publication year - 2015
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.14-0154-oa
Subject(s) - comet assay , dna damage , comet tail , toxicology , medicine , comet , occupational exposure , nuclear medicine , dna , pathology , biology , emergency medicine , physics , genetics , quantum mechanics , magnetic field , solar wind , astrobiology
Evaluation of DNA damage in lymphocytes of radiology personnel by comet assay: Muhammad K hisroon , et al . Department of Zoology, University of Peshawar, PakistanObjective The importance of X‐rays as a diagnostic medical tool cannot be denied. However, continuous exposure to X‐rays can cause DNA damage. This study aimed to use the comet assay technique to investigate the level of DNA damage in lymphocytes due to X‐rays in occupationally exposed personnel. Methods Blood samples were collected from 74 exposed and 70 control subjects for analysis. A total of 100 randomly captured cells from each slide were examined using an epifluorescent microscope. The comets were analyzed by a visual scoring method according to comet tail length. Results The results indicated a significant increase ( p <0.05) in DNA damage in X‐rays technicians (129.8 ± 17.2) as compared with the control group (53.0 ± 25.0). A significant increase ( p <0.02) in DNA damage was also observed with an increase in exposure duration of technicians because of their service length. Conclusions The present study suggests that the exposed radiology personnel should carefully comply with radiation protection procedures such as wearing of lead apron during diagnostic procedures and minimize radiation exposure where possible to avoid potential genotoxic effects due to X‐rays.