Open AccessDetermination Method for Mono‐ and Diethanolamine in Workplace Air by High‐performance Liquid Chromatography
Author(s) -
Takeuchi Akito,
Kitade Takashi,
Jukurogi Akira,
Hendricks Warren,
Kaifuku Yuichiro,
Shibayama Kenji,
Natsumeda Shuichiro,
Ota Hirokazu,
Yamada Shu,
Sumino Kimiaki,
Namera Akira,
Kanno Seiichiro
Publication year - 2012
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.12-0064-br
Subject(s) - diethanolamine , chromatography , detection limit , reproducibility , amine gas treating , chemistry , environmental science , environmental engineering , organic chemistry
Determination Method for Mono‐ and Diethanolamine in Workplace Air by High‐performance Liquid Chromatography: Akito TAKEUCHI, et al . Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association—Objectives The purpose of this research was to develop a simultaneous determination method for monoethanolamine (MEA) and diethanolamine (DEA) in workplace air for risk assessment. Methods The characteristics of the proposed method, such as recovery, quantitation limit, reproducibility and storage stability of the samples, were examined. Results An air sampling cassette containing two sulfuric acid‐treated glass fiber filters was chosen as the sampler. The MEA and DEA were extracted from the sampler filters, derivatized with 9fluorenylmethyloxycarbonyl chloride and then analyzed by a high‐performance liquid chromatograph equipped with a fluorescence detector or photo‐diode array detector. The overall recoveries from spiked samplers were 86–99 and 88–99% for MEA and DEA, respectively. The recovery after 5 days of storage in a refrigerator exceeded 95%. The overall limits of quantitation were 0.750 and 0.100 μ g/sample for MEA and DEA, respectively. The relative standard deviations, which represent the overall reproducibility defined as precision, were 0.3–1.6 and 0.4–5.7% for MEA and DEA, respectively. Conclusions The proposed method enables 4‐h personal exposure monitoring of MEA and DEA at concentrations equaling 1/3,000–2 times the threshold limit value‐time‐weighted average (TLV‐TWA: 3 ppm for MEA, 1 mg/m 3 for DEA) adopted by the American Conference of Governmental Industrial Hygienists and also by the Japan Society for Occupational Health. The method is useful for estimating worker exposure to MEA and DEA.