Open AccessPulmonary Toxicity Following an Intratracheal Instillation of Nickel Oxide Nanoparticle Agglomerates
Author(s) -
Morimoto Yasuo,
Hirohashi Masami,
Ogami Akira,
Oyabu Takako,
Myojo Toshihiko,
Hashiba Masayoshi,
Mizuguchi Yohei,
Kambara Tatsunori,
Lee Byeong Woo,
Kuroda Etsushi,
Tanaka Isamu
Publication year - 2011
Publication title -
journal of occupational health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 59
ISSN - 1348-9585
DOI - 10.1539/joh.11-0034-br
Subject(s) - intratracheal instillation , bronchoalveolar lavage , saline , toxicity , lung , nickel oxide , agglomerate , nanoparticle , chemistry , pulmonary toxicity , nickel , oxide , alveolar macrophage , inhalation , medicine , pharmacology , nuclear chemistry , pathology , immunology , macrophage , materials science , anesthesia , biochemistry , nanotechnology , in vitro , organic chemistry , composite material
Pulmonary Toxicity Following an Intratracheal Instillation of Nickel Oxide Nanoparticle Agglomerates: Yasuo Morimoto, et al. Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan—Objective We examined the pulmonary toxicity of nickel oxide nanoparticle agglomerates in the rat lung following an intratracheal instillation. Methods The weighted average surface primary diameter of nickel oxide nanoparticles was 8.41 nm, and the count median diameter of nickel oxide nanoparticle agglomerates suspended in saline was 1.34 µm. Male Wistar rats were exposed to 1 mg (3.3 mg/kg) of nickel oxide nanoparticles intratracheally. The control group Received intratracheal instillation of saline. Rats were dissected 3 days, 1 wk, 1 mo, 3 mo, and 6 mo after the instillation. Cytokine‐induced neutrophil chemoattractant (CINC)2α β in the lung tissue was determined by quantitative measurement of protein by ELISA. Results The total cell count in bronchoalveolar lavage luid (BALF) was increased persistently from 3 days to 6 mo. The neutrophil counts in BALF were also increased at 3days, 1 wk, 3 mo, and 6 mo. In the lung tissue, iniltration of mainly neutrophils and alveolar macrophages was observed in alveoli from 3 days to 6 mo. The CINC‐2α β concentration was elevated from 3 days to 6 mo in the lung tissue. Conclusions These results showed that micron‐sized nickel oxide nanoparticle agglomerates also induced a persistent inlammatory response.