Open AccessComparative evaluation of the effect of decontamination protocol on the shear bond strength of eighth generation bonding agent to contaminated dentin: an in vitro study
Author(s) -
Manasi Kewlani,
Suparna Ganguly Saha,
Anuj Bhardwaj,
Mainak Kanti Saha,
Prashansa Vijaywargiya,
Shikha Jain,
Sm Vignesh Prasad
Publication year - 2020
Publication title -
medicine and pharmacy reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.35
H-Index - 16
eISSN - 2668-0572
pISSN - 2602-0807
DOI - 10.15386/mpr-1488
Subject(s) - sodium hypochlorite , human decontamination , saliva , bond strength , dentin , chemistry , sodium ascorbate , dentistry , materials science , composite material , adhesive , food science , ascorbic acid , biochemistry , medicine , organic chemistry , pathology , layer (electronics)
Introduction. This in vitro study aims to evaluate and compare the effect of decontamination protocol - based on sodium hypochlorite - NaOCl, as a decontaminating agent, followed by the application of a collagen cross-linking agent like sodium ascorbate [Na Ascorbate] or Proanthocyanidin [PA]) on the shear bond strength of eighth generation bonding agent to contaminated dentin (saliva, blood and blood followed by hemostatic agent).
Methodology. The buccal surfaces of extracted premolars were flattened to expose the dentine. The teeth were then randomly divided into 3 groups based on the contaminant used (saliva, blood and blood followed by hemostatic agent). Decontamination protocol was carried out with sodium hypochlorite followed by application of either of the two collagen cross-linking agents (sodium ascorbate/proanthocyanidin) in each group. FuturaBond DC (eighth generation) bonding agent was used to bond composite to dentin surfaces which was subjected to shear bond strength testing. Data was analyzed by one-way ANOVA and Tukey test.
Results All the groups (contaminated with saliva, blood, or hemostatic agent) showed a decrease in shear bond strength of composite resin to dentin. The shear bond strength of composite resin to dentin was least affected by contamination with saliva followed by contamination with blood and was most affected by contamination with a hemostatic agent.
The decontamination protocol carried out showed that the combination of NaOCl and Proanthocyanidin improved the shear bond strength of composite to dentin significantly more than the combination of NaOCl and sodium ascorbate or ofsodium hypochlorite used alone.