Single-Copy Knock-In Loci for Defined Gene Expression inCaenorhabditis elegans | Zendy

Carlos G. Silva-García | Zendy; Anne Lanjuin | Zendy; Caroline Heintz | Zendy; Sneha Dutta | Zendy; Nicole M Clark | Zendy; William B. Mair | Zendy

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Single-Copy Knock-In Loci for Defined Gene Expression inCaenorhabditis elegans

Author(s) -

Carlos G. Silva-García,

Anne Lanjuin,

Caroline Heintz,

Sneha Dutta,

Nicole M Clark,

William B. Mair

Publication year - 2019

Publication title -

g3 genes genomes genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.468

H-Index - 66

ISSN - 2160-1836

DOI - 10.1534/g3.119.400314

Subject(s) - caenorhabditis elegans , biology , genetics , gene , gene knockin , gene expression

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans .

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