Efficient Homologous Recombination in Mice Using Long Single Stranded DNA and CRISPR Cas9 Nickase | Zendy

Xi Ge | Zendy; Craig P. Hunter | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Efficient Homologous Recombination in Mice Using Long Single Stranded DNA and CRISPR Cas9 Nickase

Author(s) -

Xi Ge,

Craig P. Hunter

Publication year - 2018

Publication title -

g3 genes genomes genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.468

H-Index - 66

ISSN - 2160-1836

DOI - 10.1534/g3.118.200758

Subject(s) - crispr , homologous recombination , cas9 , biology , dna , genome editing , homologous chromosome , dna repair , non homologous end joining , genetics , homology directed repair , microbiology and biotechnology , nucleotide excision repair , gene

The CRISPR/Cas9 nickase mutant is less prone to off-target double-strand (ds)DNA breaks than wild-type Cas9 because to produce dsDNA cleavage it requires two guide RNAs to target the nickase to nearby opposing strands. Like wild-type Cas9 lesions, these staggered lesions are repaired by either non-homologous end joining or, if a repair template is provided, by homologous recombination (HR). Here, we report very efficient (up to 100%) recovery of heterozygous insertions in Mus musculus produced by long (>300 nt), single-stranded DNA donor template-guided repair of paired-nickase lesions.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research