Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection | Zendy

Dawn E. WatkinsChow | Zendy; Gaurav K. Varshney | Zendy; Lisa Garrett | Zendy; Zelin Chen | Zendy; Erin Jimenez | Zendy; Cecilia Rivas | Zendy; Kevin Bishop | Zendy; Raman Sood | Zendy; Ursula L. Harper | Zendy; William J. Pavan | Zendy; Shawn M. Burgess | Zendy

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Highly Efficient Cpf1-Mediated Gene Targeting in Mice Following High Concentration Pronuclear Injection

Author(s) -

Dawn E. WatkinsChow,

Gaurav K. Varshney,

Lisa Garrett,

Zelin Chen,

Erin Jimenez,

Cecilia Rivas,

Kevin Bishop,

Raman Sood,

Ursula L. Harper,

William J. Pavan,

Shawn M. Burgess

Publication year - 2017

Publication title -

g3 genes genomes genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.468

H-Index - 66

ISSN - 2160-1836

DOI - 10.1534/g3.116.038091

Subject(s) - nuclease , cas9 , gene , crispr , biology , computational biology , microbiology and biotechnology , chemistry , cancer research , genetics

Cpf1 has emerged as an alternative to the Cas9 RNA-guided nuclease. Here we show that gene targeting rates in mice using Cpf1 can meet, or even surpass, Cas9 targeting rates (approaching 100% targeting), but require higher concentrations of mRNA and guide. We also demonstrate that coinjecting two guides with close targeting sites can result in synergistic genomic cutting, even if one of the guides has minimal cutting activity.

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