Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton | Zendy

Weina Wang | Zendy; Yǒulù Yuán | Zendy; Can Yang | Zendy; Shuaipeng Geng | Zendy; Quan Sun | Zendy; Lu Long | Zendy; Chaowei Cai | Zendy; Zongyan Chu | Zendy; Xin Liu | Zendy; Guanghao Wang | Zendy; Xiongming Du | Zendy; Chen Miao | Zendy; Xiao Zhang | Zendy; Yingfan Cai | Zendy

Open Access

Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton

Author(s) -

Weina Wang,

Yǒulù Yuán,

Can Yang,

Shuaipeng Geng,

Quan Sun,

Lu Long,

Chaowei Cai,

Zongyan Chu,

Xin Liu,

Guanghao Wang,

Xiongming Du,

Chen Miao,

Xiao Zhang,

Yingfan Cai

Publication year - 2016

Publication title -

g3 genes genomes genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.468

H-Index - 66

ISSN - 2160-1836

DOI - 10.1534/g3.116.034512

Subject(s) - verticillium wilt , verticillium dahliae , biology , abiotic stress , abiotic component , arabidopsis , gene , gossypium barbadense , gossypium , verticillium , botany , plant disease resistance , genetics , mutant , ecology

Elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. In this study, a gene containing the NAC domain, designated GbNAC1, was identified from Gossypium barbadense L. Homologous sequence alignment indicated that GbNAC1 belongs to the TERN subgroup. GbNAC1 protein localized to the cell nucleus. GbNAC1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. Functional analysis showed that cotton resistance to Verticillium wilt was reduced when the GbNAC1 gene was silenced using the virus-induced gene silencing (VIGS) method. GbNAC1-overexpressing Arabidopsis showed enhanced resistance to Verticillium dahliae compared to wild-type. Thus, GbNAC1 is involved in the positive regulation of resistance to Verticillium wilt. In addition, analysis of GbNAC1-overexpressing Arabidopsis under different stress treatments indicated that it is involved in plant growth, development, and response to various abiotic stresses (ABA, mannitol, and NaCl). This suggests that GbNAC1 plays an important role in resistance to biotic and abiotic stresses in cotton. This study provides a foundation for further study of the function of NAC genes in cotton and other plants.

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