Open AccessA Forward Genetic Screen for Suppressors of Somatic P Granules inCaenorhabditis elegans
Author(s) -
Ashley L. Kelly,
Michael Senter-Zapata,
Anne C. Campbell,
Hannah E Lust,
Monique E. Theriault,
Karolina M. Andralojc,
Dustin L. Updike
Publication year - 2015
Publication title -
g3 genes genomes genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.468
H-Index - 66
ISSN - 2160-1836
DOI - 10.1534/g3.115.019257
Subject(s) - caenorhabditis elegans , biology , germline , somatic cell , genetics , allele , retinoblastoma , chromatin , phenotype , genetic screen , germline mutation , ectopic expression , gene , microbiology and biotechnology , mutation
In Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs of tumor suppressors Retinoblastoma (Rb/LIN-35) and Malignant Brain Tumor (MBT/LIN-61). However, the full scope of pathways that suppress germline expression in the soma is unknown. To address this, we performed a mutagenesis and screened for somatic expression of GFP-tagged PGL-1, a core P-granule nucleating protein. Eight alleles were isolated from 4000 haploid genomes. Five of these alleles exhibit a synMuv phenotype, whereas the remaining three were identified as hypomorphic alleles of known synMuv B genes, lin-13 and dpl-1. These findings suggest that most suppressors of germline programs in the soma of C. elegans are either required for viability or function through synMuv B chromatin regulation.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom