Open Access
Estimation of seed viability constants (KE,CW) for lentil (Lens culinaris)
Author(s) -
Katherine J. Whitehouse,
Sally L. Norton
Publication year - 2022
Publication title -
seed science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.246
H-Index - 42
eISSN - 1819-5717
pISSN - 0251-0952
DOI - 10.15258/sst.2022.50.1.09
Subject(s) - longevity , germination , water content , biology , moisture , horticulture , botany , materials science , engineering , composite material , genetics , geotechnical engineering
Orthodox seed longevity systematically increases with reduction in temperature and moisture content, as described by the Ellis and Roberts viability equations. These equations have species-specific parameters which quantify the inherent longevity of seeds of a particular species and their sensitivity to change in moisture content. This research investigated the effect of moisture content on lentil ( Lens culinaris ) seed longevity during experimental storage. Seeds of an elite breeding line were stored under hermetic conditions at six different moisture contents (7.6, 8.1, 8.8, 10.1, 11.5, 12.6%) at 60°C. Ability to germinate was determined at scheduled intervals, and longevity (σ) was estimated for each treatment. The values of the constants K E (8.0008) and C W (3.746) were estimated using linear regression, by plotting log σ against log moisture content, combined with the universal temperature constants ( C H = 0.0329, C Q = 0.000478). The application of these moisture constants ( K E and C W ) was tested by comparing the predicted viability loss of two lentil accessions with their observed longevity under hermetic storage at 12.0% moisture content and 45°C. The validity of these constants for predicting lentil seed longevity was proven, with an R 2 = 0.912 ( P < 0.001).