Open AccessMicrobial communities of vegetable seeds and biocontrol microbes for seed treatment
Author(s) -
Tinatin Döölotkeldieva,
Saykal Bobusheva
Publication year - 2022
Publication title -
seed science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.246
H-Index - 42
eISSN - 1819-5717
pISSN - 0251-0952
DOI - 10.15258/sst.2022.50.1.08
Subject(s) - biology , pectobacterium carotovorum , pseudomonas , germination , xanthomonas , erwinia , pseudomonas syringae , botany , agar plate , pectobacterium , 16s ribosomal rna , horticulture , bacteria , microbiology and biotechnology , pathogen , genetics
Thirty-one species of vegetable seeds from Kyrgyzstan's State Register were analysed for mycological and bacterial composition using various methods, from simple visual to molecular. The modified roll-towel method simultaneously assesses the degree of contamination and seed germination. S creening in agar medium allows analysis of species on the outer and inner surfaces of vegetable seeds that are not detected using the roll-towel method. As a result of the phytopathological examination, 15 fungal and three bacterial species were found. Direct DNA extraction and amplification of the universal 16S rRNA gene of diseased seeds was used to identify the plant pathogenic bacteria, which included Pseudomonas syringae , Pseudomonas savastanoi , Pseudomonas cerasi , Pectobacterium carotovorum , Xanthomonas oryzae , Erwinia sp. and others from the Proteobacteria phylum. For the coating of vegetable seeds, S. alfalfae CI-4 was selected. This strain had a beneficial double effect on the tested vegetable seeds at 1 × 10 3 spores mL -1 .