Open AccessHydropriming enhances the germination of aged ultra-dry wheat seeds
Author(s) -
Bowen Jiang,
Liyuan Wang,
Chengtang Xu,
Min Yan
Publication year - 2020
Publication title -
seed science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.246
H-Index - 42
eISSN - 1819-5717
pISSN - 0251-0952
DOI - 10.15258/sst.2020.48.1.08
Subject(s) - germination , hydrogen peroxide , catalase , malondialdehyde , amylase , distilled water , priming (agriculture) , biology , horticulture , moisture , aeration , food science , agronomy , chemistry , enzyme , oxidative stress , biochemistry , ecology , organic chemistry , chromatography
Hydropriming is an efficient strategy for reviving aged seeds. In the present study, hydropriming was optimised for vigour enhancement in aged ultra-dry wheat seeds. Wheat seeds (initially dried to 5.0% moisture content) stored at ambient temperature for ten years were humidified over water for seven days, then soaked for 12, 24 and 36 hours in aerated distilled water (seed priming treatment). Primed and unprimed seeds were equilibrated at ambient conditions for 48 hours. Changes in germination traits, activities of anti-oxidative enzymes, hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), α-amylase activity, soluble protein and soluble sugars were determined. Results indicated that hydro priming for 12 hours significantly increased the germination percentage, from 50.7 to 58.6%. The increase in germination was significantly positively correlated with catalase activity ( r = 0.969), and significantly negatively correlated with accumulation of H 2 O 2 ( r = 1.000).