Open AccessAcidovorax citrulli is sensitive to elevated temperatures during early stages of watermelon seed germination
Author(s) -
Mei Zhao,
R. R. Walcott
Publication year - 2020
Publication title -
seed science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.246
H-Index - 42
eISSN - 1819-5717
pISSN - 0251-0952
DOI - 10.15258/sst.2020.48.1.02
Subject(s) - germination , seedling , biology , sowing , horticulture , population , relative humidity , botany , agronomy , physics , demography , sociology , thermodynamics
Bacterial fruit blotch (BFB), caused by Acidovorax citrulli , is a seed-transmitted disease of cucurbit crop species. During seed-to-seedling transmission of BFB, A. citrulli initially grows as a saprophyte on germinating seeds and subsequently switches to a pathogenic mode. We investigated the effect of temperature on A. citrulli colonisation of germinating watermelon seeds. Seeds were vacuum-infiltrated with 10 6 CFU/ml A. citrulli , germinated at 28°C and 100% relative humidity, and transferred to 40°C at different times. Mean BFB incidence was significantly lower for seeds that were sown at 28°C and transferred to 40°C three days after sowing (DAS), compared with seeds incubated constantly at 28°C. Seeds showed reduced mean BFB transmission percentages when transferred from 28 to 40°C at 3 DAS, regardless of initial A. citrulli concentration. The effect of increased temperature on BFB seedling transmission was reversible regardless of the initial A. citrulli inoculum concentration. Furthermore, the A. citrulli population on germinating watermelon seedlings that were transferred from 28 to 40°C at 3 DAS was significantly lower than seedlings maintained at 28°C. We conclude that A. citrulli cells associated with germinating watermelon seeds are more sensitive to elevated temperature during the first 3 DAS relative to the later days.