Open AccessA solid‐phase transfection platform for arrayed CRISPR screens
Author(s) -
Serçin Özdemirhan,
Reither Sabine,
Roidos Paris,
Ballin Nadja,
Palikyras Spyridon,
Baginska Anna,
Rein Katrin,
Llamazares Maria,
Halavatyi Aliaksandr,
Winter Hauke,
Muley Thomas,
Jurkowska Renata Z,
Abdollahi Amir,
Zenke Frank T,
Neumann Beate,
Mardin Balca R
Publication year - 2019
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.15252/msb.20198983
Subject(s) - center (category theory) , library science , german , west germany , chemistry , computer science , philosophy , history , linguistics , economic history , crystallography
Arrayed CRISPR ‐based screens emerge as a powerful alternative to pooled screens making it possible to investigate a wide range of cellular phenotypes that are typically not amenable to pooled screens. Here, we describe a solid‐phase transfection platform that enables CRISPR ‐based genetic screens in arrayed format with flexible readouts. We demonstrate efficient gene knockout upon delivery of guide RNA s and Cas9/guide RNA ribonucleoprotein complexes into untransformed and cancer cell lines. In addition, we provide evidence that our platform can be easily adapted to high‐throughput screens and we use this approach to study oncogene addiction in tumor cells. Finally demonstrating that the human primary cells can also be edited using this method, we pave the way for rapid testing of potential targeted therapies.