Open AccessA deep proteome and transcriptome abundance atlas of 29 healthy human tissues
Author(s) -
Wang Dongxue,
Eraslan Basak,
Wieland Thomas,
Hallström Björn,
Hopf Thomas,
Zolg Daniel Paul,
Zecha Jana,
Asplund Anna,
Li Lihua,
Meng Chen,
Frejno Martin,
Schmidt Tobias,
Schnatbaum Karsten,
Wilhelm Mathias,
Ponten Frederik,
Uhlen Mathias,
Gagneur Julien,
Hahne Hannes,
Kuster Bernhard
Publication year - 2019
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.15252/msb.20188503
Subject(s) - biology , transcriptome , proteome , computational biology , atlas (anatomy) , abundance (ecology) , bioinformatics , gene expression , genetics , ecology , gene , anatomy
Abstract Genome‐, transcriptome‐ and proteome‐wide measurements provide insights into how biological systems are regulated. However, fundamental aspects relating to which human proteins exist, where they are expressed and in which quantities are not fully understood. Therefore, we generated a quantitative proteome and transcriptome abundance atlas of 29 paired healthy human tissues from the Human Protein Atlas project representing human genes by 18,072 transcripts and 13,640 proteins including 37 without prior protein‐level evidence. The analysis revealed that hundreds of proteins, particularly in testis, could not be detected even for highly expressed mRNA s, that few proteins show tissue‐specific expression, that strong differences between mRNA and protein quantities within and across tissues exist and that protein expression is often more stable across tissues than that of transcripts. Only 238 of 9,848 amino acid variants found by exome sequencing could be confidently detected at the protein level showing that proteogenomics remains challenging, needs better computational methods and requires rigorous validation. Many uses of this resource can be envisaged including the study of gene/protein expression regulation and biomarker specificity evaluation.