Open AccessExtensive mapping of an innate immune network with CRISPR
Author(s) -
Aregger Michael,
Hart Traver,
Moffat Jason
Publication year - 2015
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.15252/msb.20156373
Subject(s) - biology , crispr , innate immune system , computational biology , immune system , genetics , gene
The application of the CRISPR ‐Cas9 system marks a major breakthrough for genetic screens, particularly in mammalian cells where high‐throughput targeted gene editing has been lacking. Parnas et al ([Parnas O, 2015]) apply this screening technology to mouse bone marrow‐derived dendritic cells in order to study the regulation of the immune response triggered by PAMP s. Through integrated analysis of gene knockouts in conjunction with changes in protein and mRNA expression, CRISPR screens are facilitating dissection of immune regulatory networks at unprecedented resolution.