Open AccessEfficient sample processing for proteomics applications—Are we there yet?
Author(s) -
Kanshin Evgeny,
Thibault Pierre
Publication year - 2014
Publication title -
molecular systems biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 8.523
H-Index - 148
ISSN - 1744-4292
DOI - 10.15252/msb.20145760
Subject(s) - proteomics , biology , computational biology , sample (material) , quantitative proteomics , throughput , scalability , computer science , chromatography , genetics , telecommunications , database , chemistry , gene , wireless
The ability to solubilize and digest protein extracts and recover peptides with high efficiency is of paramount importance in proteomics. A novel proteomic sample preparation protocol by Krijgsveld and colleagues (Hughes et al, 2014) provides significant advantages by enabling all sample processing steps to be carried out in a single tube to minimize sample losses, thereby enhancing sensitivity, throughput, and scalability of proteomics analyses.