Open AccessA Parkinson's disease Circ RNA s Resource reveals a link between circ SLC 8A1 and oxidative stress
Author(s) -
Hanan Mor,
Simchovitz Alon,
Yayon Nadav,
Vaknine Shani,
CohenFultheim Roni,
Karmon Miriam,
Madrer Nimrod,
Rohrlich Talia Miriam,
Maman Moria,
Bennett Estelle R,
Greenberg David S,
Meshorer Eran,
Leva Erez Y,
Soreq Hermona,
Kadener Sebastian
Publication year - 2020
Publication title -
embo molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.923
H-Index - 107
eISSN - 1757-4684
pISSN - 1757-4676
DOI - 10.15252/emmm.201911942
Subject(s) - hebrew , chinese academy of sciences , theology , chemistry , classics , history , philosophy , china , archaeology
Circular RNA s (circ RNA s) are brain‐abundant RNA s of mostly unknown functions. To seek their roles in Parkinson's disease ( PD ), we generated an RNA sequencing resource of several brain region tissues from dozens of PD and control donors. In the healthy substantia nigra ( SN ), circ RNA s accumulate in an age‐dependent manner, but in the PD SN this correlation is lost and the total number of circ RNA s reduced. In contrast, the levels of circ RNA s are increased in the other studied brain regions of PD patients. We also found circ SLC 8A1 to increase in the SN of PD individuals. Circ SLC 8A1 carries 7 binding sites for miR‐128 and is strongly bound to the micro RNA effector protein Ago2. Indeed, RNA targets of miR‐128 are also increased in PD individuals, suggesting that circ SLC 8A1 regulates miR‐128 function and/or activity. Circ SLC 8A1 levels also increased in cultured cells exposed to the oxidative stress‐inducing agent paraquat but were decreased in cells treated with the neuroprotective antioxidant regulator drug Simvastatin. Together, our work links circ SLC 8A1 to oxidative stress‐related Parkinsonism and suggests further exploration of its molecular function in PD .