Open AccessCobalt protoporphyrin IX increases endogenous G‐ CSF and mobilizes HSC and granulocytes to the blood
Author(s) -
Szade Agata,
Szade Krzysztof,
Nowak Witold N,
BukowskaStrakova Karolina,
Muchova Lucie,
Gońka Monika,
Żukowska Monika,
Cieśla Maciej,
KachamakovaTrojanowska Neli,
RamsBaron Marzena,
Ratuszna Alicja,
Dulak Józef,
Józkowicz Alicja
Publication year - 2019
Publication title -
embo molecular medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.923
H-Index - 107
eISSN - 1757-4684
pISSN - 1757-4676
DOI - 10.15252/emmm.201809571
Subject(s) - library science , chemistry , computer science
Granulocyte colony‐stimulating factor (G‐ CSF ) is used in clinical practice to mobilize cells from the bone marrow to the blood; however, it is not always effective. We show that cobalt protoporphyrin IX (Co PP ) increases plasma concentrations of G‐ CSF , IL ‐6, and MCP ‐1 in mice, triggering the mobilization of granulocytes and hematopoietic stem and progenitor cells ( HSPC ). Compared with recombinant G‐ CSF , Co PP mobilizes higher number of HSPC and mature granulocytes. In contrast to G‐ CSF , Co PP does not increase the number of circulating T cells. Transplantation of Co PP ‐mobilized peripheral blood mononuclear cells ( PBMC ) results in higher chimerism and faster hematopoietic reconstitution than transplantation of PBMC mobilized by G‐ CSF . Although Co PP is used to activate Nrf2/ HO ‐1 axis, the observed effects are Nrf2/ HO ‐1 independent. Concluding, Co PP increases expression of mobilization‐related cytokines and has superior mobilizing efficiency compared with recombinant G‐ CSF . This observation could lead to the development of new strategies for the treatment of neutropenia and HSPC transplantation.